Journal: Surgery

Article Title: miR-335 and miR-363 REGULATION OF NEUROBLASTOMA TUMORIGENESIS AND METASTASIS

doi: 10.1016/j.surg.2013.04.005

Figure Lengend Snippet: (A) Cell proliferation was measured by plating cells in 96-well plates at a density of 5 × 103 cells per well in RPMI 1640 culture media with 10% FBS and grown for up to 96 h posttransfection. Cell number was assessed daily using Cell-Counting Kit-8. (B) Cell clonogenicity assay was performed by plating cells at low density in 6-well plates (1 × 103 cells/well) and incubated for 10 days. Colonies were fixed and stained with 0.005% crystal violet in 70% methanol for overnight (*= p <0.05 vs. miR-CON). (C) Anchorage-independent growth was examined by culturing cells in 12-well plates (2 × 103 cells/well) with 0.4 % agarose and incubated for 2 weeks prior to staining with 0.005% crystal violet. The colony images were taken with Bio-Rad Gel Doc XR+ Imaging System, and quantitated using Bio-Rad Quantity One Gel Doc version 4.6.9 software (*= p <0.05 vs. miR-CON).

Article Snippet: Quantity One Gel Doc version 4.6.9 software from Bio-Rad was used to count the colonies.

Techniques: Cell Counting, Incubation, Staining, Imaging, Software